Analytical comparison of in vitro spiked human serum and plasma for the PCR - based 1 detection of Aspergillus fumigatus DNA – a study by the European Aspergillus PCR
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چکیده
Analytical comparison of in vitro spiked human serum and plasma for the PCR-based 1 detection of Aspergillus fumigatus DNA – a study by the European Aspergillus PCR 2 Initiative 3 4 Juergen Loeffler*, Carlo Mengoli, Jan Springer, Stephane Bretagne, Manuel Cuenca 5 Estrella, Lena Klingspor, Katrien Lagrou, Willem JG Melchers, C. Oliver Morton, 6 Rosemary A. Barnes, J. Peter Donnelly, and P. Lewis White, on behalf of the European 7 Aspergillus PCR Initiative 8 9 Affiliations: Wuerzburg University, Wuerzburg, Germany, University of Padua, Padua, 10 Italy, Paris Diderot University, Saint Louis Hospital-APHP, Paris, France; Spanish National 11 Centre for Microbiology, Instituto de Salud Carlos III, Madrid, Spain; Karolinska University 12 Hospital, Stockholm, Sweden; National Reference Center for Mycosis, University Hospitals 13 Leuven, Leuven, Belgium and Department of Microbiology and Immunology, KU Leuven, 14 Leuven, Belgium; Radboud University Medical Centre, Nijmegen, The Netherlands, 15 University of Western Sydney, Sydney, NSW, Australia; Cardiff University, UHW, Cardiff; 16 Public Health Wales Microbiology Cardiff, UK. 17 18 *Correspondence 19 Prof. Dr. Jürgen Löffler 20 University Hospital Wuerzburg 21 Medical Hospital II, Building C11 22 97080 Wuerzburg, Germany 23 Telephone +49 931 20136412, Fax: +49 931 20136409 24 Mailto: [email protected] 25 JCM Accepted Manuscript Posted Online 17 June 2015 J. Clin. Microbiol. doi:10.1128/JCM.00906-15 Copyright © 2015, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Analytical Comparison of In Vitro-Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative
The use of serum or plasma for Aspergillus PCR testing facilitates automated and standardized technology. Recommendations for serum testing are available, and while serum and plasma are regularly considered interchangeable for use in fungal diagnostics, differences in galactomannan enzyme immunoassay (GM-EIA) performance have been reported and are attributed to clot formation. Therefore, it is ...
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تاریخ انتشار 2015